Coronavirus Controls qPCR probes and Primers • COVID-19 Diagnostics • MultiplexDX

‍Primer and Probe Sets from National Institute For Viral Disease Control and Prevention / China

Name	Description	Oligonucleotide Sequence (5’>3’)
2019-nCoV_ORF1ab-F	2019-nCoV_ORF1ab Forward Primer	5’-CCC TGT GGG TTT TAC ACT TAA-3’
2019-nCoV_ORF1ab-R	2019-nCoV_ORF1ab Reverse Primer	5’-ACG ATT GTG CAT CAG CTGA-3’
2019-nCoV_ORF1ab-P	2019-nCoV_ORF1ab Probe	5’-FAM-CCG TCT GCG GTA TGT GGA AAG GTT ATG G-BHQ1-3’
2019-nCoV_N-F	2019-nCoV_N Forward Primer	5’-GGG GAA CTT CTC CTG CTA GAA T-3’
2019-nCoV_N-R	2019-nCoV_N Reverse Primer	5’-CAG ACA TTT TGC TCT CAA GCT G-3’
2019-nCoV_N-P	2019-nCoV_N Probe	5’-FAM-TTG CTG CTG CTT GAC AGA TT-BHQ1-3’

Primer and Probe Sets from Centers for Disease Control and Prevention / CDC, USA

(see reference)

Name	Description	Oligonucleotide Sequence (5’>3’)
2019-nCoV_N1-F	2019-nCoV_N1 Forward Primer	5’-GAC CCC AAA ATC AGC GAA AT-3’
2019-nCoV_N1-R	2019-nCoV_N1 Reverse Primer	5’-TCT GGT TAC TGC CAG TTG AAT CTG-3’
2019-nCoV_N1-P	2019-nCoV_N1 Probe	5’-FAM-ACC CCG CAT TAC GTT TGG TGG ACC-BHQ1-3’
2019-nCoV_N2-F	2019-nCoV_N2 Forward Primer	5’-TTA CAA ACA TTG GCC GCA AA-3’
2019-nCoV_N2-R	2019-nCoV_N2 Reverse Primer	5’-GCG CGA CAT TCC GAA GAA-3’
2019-nCoV_N2-P	2019-nCoV_N2 Probe	5’-FAM-ACA ATT TGC CCC CAG CGC TTC AG-BHQ1-3’
2019-nCoV_N3-F	2019-nCoV_N3 Forward Primer	5’-GGG AGC CTT GAA TAC ACC AAA A-3’
2019-nCoV_N3-R	2019-nCoV_N3 Reverse Primer	5’-TGT AGC ACG ATT GCA GCA TTG-3’
2019-nCoV_N3-P	2019-nCoV_N3 Probe	5’-FAM-AYC ACA TTG GCA CCC GCA ATC CTG-BHQ1-3’
RP-F	RNAse P Forward Primer	5’-AGA TTT GGA CCT GCG AGC G-3’
RP-R	RNAse P Reverse Primer	5’-GAG CGG CTG TCT CCA CAA GT-3’
RP-P	RNAse P Probe	5’-FAM-TTC TGA CCT GAA GGC TCT GCG CG-BHQ-1-3’

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RECOMMENDATION:

‍

To achieve the highest accuracy of virus testing, it is necessary to consider that:

The CDC primers for SARS-CoV-2 have a lot of background (see reference)
The SARS-CoV-2 has almost 90% similarity to other SARS-like coronaviruses, which can cause confusion in detecting the right virus (see reference)

For the most accurate qPCR results:
1) We will synthesize custom primers and probes of your choice
2) We will offer design of the most specific primers and TaqMan probes

AMOUNTS:

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For 500 rxn kit, we offer 10 nmol of reverse and forward primer and 2.5 nmol of TaqMan probe.

PRICE:

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MDX SARS-CoV-2 RUO Kit, 100 rxn - €49,00

MDX SARS-CoV-2 RUO Kit, 500 rxn - €98,00

MDX SARS-CoV-2 4xTarget Kit, 500 rxn - €380,00

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RT-PCR Control Accessory Kit

‍Primer and Probe Sets from National Institute For Viral Disease Control and Prevention / China

Primer and Probe Sets from Centers for Disease Control and Prevention / CDC, USA

RECOMMENDATION:

AMOUNTS:

PRICE:

FAQs

PRODUCT ORDER / SUPPORT / ENQUIRY

ENQUIRY ABOUT PRODUCT

CONTROLS, qPCR PROBES AND PRIMERS

ENQUIRY ABOUT MULTIPLEX8+

REQUEST YOUR FREE

FullRNAlook SAMPLE KIT

RT-PCR Control Accessory Kit

‍Primer and Probe Sets from National Institute For Viral Disease Control and Prevention / China

Primer and Probe Sets from Centers for Disease Control and Prevention / CDC, USA

RECOMMENDATION:

AMOUNTS:

PRICE:

FAQs

PRODUCT ORDER / SUPPORT / ENQUIRY

ENQUIRY ABOUT PRODUCT

CONTROLS, qPCR PROBES AND PRIMERS

ENQUIRY ABOUT MULTIPLEX8+

REQUEST YOUR FREE

FullRNAlook SAMPLE KIT

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