The rTEST COVID-19 qPCR Superplex kit is an innovative, improved, and re-designed version of the WHO- recommended Charite, Berlin protocol with an external spike-in control.
The kit contains four sets of primers and hydrolysis probes (TaqMan®) targeting the SARS-CoV-2 specific E and RdRP genes, together with human RNase P or exogenous control RNA Spike-In. TaqMan® probes for E and RdRP genes are conjugated to YY and FAM, respectively, TaqMan® probes for human RNase P and RNA Spike-In are conjugated to Cy5. This enables multiplexed detection of SARS-CoV-2 and human RNase P (internal control to validate proper sample collection, RNA extraction and performance of the test) or RNA Spike-In (exogenous control to verify RNA extraction, reverse transcription, performance of the test primarily in SARS-CoV-2 negatives samples).The kit is intended exclusively for use in a diagnostic laboratory with the appropriate equipment, safety standards and properly trained personnel. One package of the kit is sufficient for 1000 tests. Multiplexing allows co-detection of SARS-CoV-2 (confirmation of positive COVID-19) and human RNase P or RNA Spike-In, which can be also used for secondary confirmation of negative samples.
Registration code: P1430A
MultiplexDX, s.r.o. IČO: 50111965 Ilkovičova 8, 841 04 Bratislava, Slovenská republika +421 2 902 68 310 info@multiplexdx.com
